| Eolas Dualfhoinse |
A commercial E. cinerea cut foliage plantation in Co. Dublin, established in 2019 and managed intensively (irrigation, fertilisation, herbicide weed control). Five trees of similar age and management history were selected at random across three production fields. From each tree, six leaves, six stem sections (about 2 cm) and six bark pieces were taken from several canopy positions and pooled by compartment. All samples were collected on one day, put on ice, and stored at -80 C. Tissues were not surface-sterilised. DNA and sequencing: total DNA was extracted with the Qiagen DNeasy Plant Mini Kit. Novogene (Cambridge, UK) amplified the fungal ITS region with primers ITS5-1737F (5'-GGAAGTAAAAGTCGTAACAAGG-3') and ITS2-2043R (5'-GCTGGTTCATTCATCGATGC-3'), prepared libraries, and sequenced them on an Illumina NovaSeq (PE150). Library negative controls produced no detectable amplification and were not sequenced. |
Derilinx