@prefix dcat: <http://www.w3.org/ns/dcat#> .
@prefix dct: <http://purl.org/dc/terms/> .
@prefix foaf: <http://xmlns.com/foaf/0.1/> .
@prefix rdfs: <http://www.w3.org/2000/01/rdf-schema#> .
@prefix skos: <http://www.w3.org/2004/02/skos/core#> .
@prefix vcard: <http://www.w3.org/2006/vcard/ns#> .
@prefix xsd: <http://www.w3.org/2001/XMLSchema#> .

<https://data.gov.ie/dataset/643b11d9-a4f7-4a63-8b2d-c6a4c4e071bd> a dcat:Dataset ;
    dct:created "2026-10-08T13:10:09"^^xsd:dateTime ;
    dct:description "Electronic supplementary material (ESM S1-S5) for a study that redeveloped a KASP marker for the H1 potato cyst nematode (PCN) resistance locus in potato. Bulked resequencing data from Meade et al. (2020) were re-analysed with the CoSSA k-mer subtraction approach. H1-associated k-mers were extracted and mapped to the DM potato reference genome, giving 99 candidate SNPs near the diagnostic 57R marker region. Two SNPs were converted to KASP assays and tested on three years of breeding material. One gave a modest but statistically significant improvement in classification accuracy over the previously published marker. The dataset documents a low-cost workflow for turning reference-free k-mer signals from public sequence data into breeder-ready marker assays. It contains: the k-mer datasets and set operations used (S1); the effect of k-mer depth thresholds on genome-wide signal and H1 enrichment (S2, S3); multi-year KASP calls with phenotypic PCN resistance data for the validation panel (S4); and the 99 candidate SNPs (S5)."@en,
        ""@ga ;
    dct:identifier "643b11d9-a4f7-4a63-8b2d-c6a4c4e071bd" ;
    dct:issued "2026-10-08T13:10:09"^^xsd:dateTime ;
    dct:language <http://publications.europa.eu/resource/authority/language/ENG> ;
    dct:modified "2026-10-08T13:10:09"^^xsd:dateTime ;
    dct:provenance [ a dct:ProvenanceStatement ;
            rdfs:label "Publicly available bulked resequencing data from Meade et al. (2020) were downloaded from the read archives (ESM S1). The new KASP assays were validated on potato breeding material across three years, with PCN resistance phenotypes. k-mers were counted in resistant and susceptible sequencing pools, and H1-associated k-mers extracted by set subtraction (CoSSA), testing several depth thresholds (ESM S2, S3). The retained k-mers were mapped to the DM reference genome to identify candidate SNPs near the 57R region (ESM S5). Two SNPs were converted to KASP assays and their classification accuracy compared with the previously published marker against phenotypic PCN resistance (ESM S4)" ] ;
    dct:publisher <https://data.gov.ie/organization/teagasc> ;
    dct:rights [ a dct:RightsStatement ;
            skos:prefLabel "https://creativecommons.org/licenses/by/4.0/deed.en" ] ;
    dct:title "KASP Marker Development for Potato H1 Resistance via Public K-mer Analysis"@en,
        ""@ga ;
    dcat:contactPoint [ a vcard:Organization ;
            vcard:fn "Stephen Byrne " ;
            vcard:hasEmail <mailto:stephen.byrne@teagasc.ie> ] ;
    dcat:keyword "K-mer analysis",
        "Molecular markers",
        "Potato",
        "Solanum tuberosum" ;
    dcat:landingPage <https://zenodo.org/records/19629845> ;
    dcat:theme <Agriculture>,
        <http://publications.europa.eu/resource/authority/data-theme/AGRI> .

<http://publications.europa.eu/resource/authority/language/ENG> a dct:LinguisticSystem .

<https://data.gov.ie/organization/teagasc> a foaf:Agent ;
    foaf:name "Teagasc" .

<https://zenodo.org/records/19629845> a foaf:Document .